MITS Engineering College-Biotechnology in Rayagada,Orissa

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BIOTECHNOLOGY DEPARTMENT

The visionaries of the MITS initiated the M. Sc. And B.Tech course in Biotechnology in the year 2002-03. The Division of Biotechnology is an independent wing of MITS & it imparts education in both science & engineering streams to meet the growing demands of the trained manpower for strong Biotechnology Industry base in the country. MITS providing two years full time course in M.Sc Biotechnology, Microbiology, Bioinformatics & Industrial Biotechnology, three years full time course in B.Sc Biotechnology (Honours) and four years full time course in B. Tech Biotechnology. MITS is the first institution to have started the B.Tech Biotechnology, M. Sc. Biotechnology and B.Sc. Biotechnology (Honours) in the entire state ofOrissa. MITS is the model Biotechnology Institution in Orissa and it has already been recognized by Biospectrum & BCIL for its quality education. MITS entered in to a MOU the British Company D1 Oils PLC, London (UK) together with the condition of placement opportunities for the students of MITS. Subsequently, a memorandum of agreement was made in collaboration with AVRDC, Geneva, Italy to establish a centre of excellence in Pulses Biotechnology.

Principal Strength of the Department

The major strength of the department is its highly motivated & distinguished workforce. The Department of Biotechnology possesses highly qualified, well trained and dedicated faculty members. The out standing performance of students at University examinations, CSIR-NET, GATE and other competitive examinations reflects the high caliber and commitment of the faculty members. Ultra modern and well equipped R & D lab and a cluster of Biogeneric industries of national and international repute.

Key Faculties

Name of the Faculty members

Position

Qualification & Distinctions

Teaching Experience

Dr. Amiya Kumar Patel

HOD

Microbiology, Molecular Biology,
Protein Engineering

7 years

Dr. (Mrs.) Anita Tripathy

Asst. Prof.

Env. Biotechnology, Biochemistry, Biostatistics

9 years

Er. P.V.S. Yugandhar

Asst. Prof.

Bioprocess engg. & Biochem. Engg.

7 years

Mr. V.S.S. Rao

Sr. Lecturer

Immunology, Animal Cell culture

8 years

Mrs. Dileswari Choudhury

Sr. Lecturer

Cell Biology & Cytogenetics

8 years

Mr. Prabhudatta Acharya

Lecturer

Medical Microbiology, Food Biotechnology

4 years

Dr. G. Raghavendrulu

Lecturer

Marine Microbiology
Env. Biotechnology

1 year

Mr. Romya Ranjan Mishra

Lecturer

Plant Biotechnology

4 years

Er. Raghunath Satapathy

Lecturer

Protein Engineering,
Drug designing, Bioinformatics

2 years

Er. Tanmay Kumar Sahoo

Lecturer

Enz. Technology, Bioinstrumentation

2 years

Ms. Nibedita Pati

Lecturer

Biochemistry

3 years

Mr. Motilal Behera

Lecturer

Plant Tissue culture

4 years

Mr. Sunil B. Maharana

Lecturer

Plant Tissue culture

3 years

Ms. Vimal Yadav

Lecturer

Bioinformatics

2 years

Er. Susant Kumar Padhi

Lecturer

Plant Biotechnology

1 year

Er. Lopa Pattanaik

Lecturer

Plant Biotechnology

1 year

Laboratories

The department has an independent two storied building and each floor measuring 28,000 sq.ft. The ground floor has state of the art laboratories for

  • Molecular Biology and Generic Engineering
  • Plant Biotechnology and Genetic Transformation
  • Bioinstrumentation
  • Microbiology and Immunology
  • Biochemistry and Enzymology
  • Bioinformatics
  • Cytogenetics
  • Fermentation Technology
  • Animal Cell Culture  

Instrumentation facilities

               All the labs are well equipped with various sophisticated instruments viz. Gene gun, PCR Machine, Gradient Thermal cycler, Gel Documentation System, Bioreactors of various design, UV-Vis spectrophotometer, EliSA reader, DNA Sequencer, 2-D gel system, Vertical and Submarine Electrophoresis apparatus, Lyophilizer,  Carbon dioxide Incubator, Laminar Airflow, Autoclave, Ultracentrifuge, Electronics balances, Shaking Incubator, BOD Incubator, Bacterial Incubator, Deep Freezer, Nanopure Water Purification Systems, Chromatography Apparatus, Sterizoomic Microscope, Binocular/ Trinocular, Microscope with photographic attachments, Flourescence Microscope, Digital Shaking baths. Bioinformatics lab is well aided with 20 Window/linux based P-IV systems with various software including GCG-WISCONSIN package, MAPMAKER, MOE, Phylip, SPSS, RASMOL, PFD-TLB, NTSYS-Pc etc. 

Aided/ Sponsored/ Collaborated Research Projects

  • Dr. J. Panigrahi, Head, Division of Biotechnology has undertaken a research project “Micropropagation of Jatropha curcas through Synthetic seed approach for Bio-Diesel Production” in collaboration” collaboration with D 1 Oils PLC, London.
  • Dr. J. Panigrahi, Head, Division of Biotechnology has undertaken a DST-FT project “Molecular characterization of somaclonal variants in Asteracantha longifolia and formulation of herbal drugs for gallstone dissolution”. It has been sanctioned by Department of Science & Technology, Govt. of India. (Rs.11.55 Lakh Only)
  • Dr. J. Panigrahi has undertaken a collaborative research project of “Genetic Fingerprinting of Eucalyptus clones using RAPD markers” with JK paper Ltd.
  • Dr. J. Panigrahi, Ms. S.P.Mishra, Mr. P.D.Achary and Mr. R. R. Mishra has undertaken a collaborative research project of “Development suitable marker system for the identification of HIV/Tuberculosis Patients” and “ Molecular Characterization of enteropathogenic strains of E. coli on epidemic outbreaks” with MKCG medical college, Berhampur.
  • Dr. J. Panigrahi, Head, Division of Biotechnology has undertaken a joint research project “Genome analysis in the genus Vigna” in collaboration with Dr. Eric Lander and Dr. N.D Young, AVRDC, Geneva, Italy.
  • Dr. J. Panigrahi, Head, Division of Biotechnology has undertaken an international funded project “Molecular mapping of genes conferring resistance to pod borer and pod fly in Cajanus cajan (L.) Millsp.”. It has been sanctioned by International Science foundation, Sweden (US$ 36,000.00 Only)
  • Mr. S. Rout, Assistant Professor, Division of Biotechnology has undertaken a collaborative research project “ Evaluation of Phramacological properties of an endangered medicinal plant- Asteracantha longifolia” in collaboration with Microbial Biotechnology and Down stream processing lab, IIT, Kharagpur. 

R & D Achievements of the Department

2004-05:

  • Molecular tagging of genes controlling seed color and flower color in solanaceae using RAPD primers.
  • Biomolecular characterization of in-vitro organogenesis in Asteracantha longifolia (L) Nees and formulation of herbal drugs for gall stone dissolution.
  • Isolation and characterization of plasmids having MCS from the bacterial strains isolated from soil/water of IMFA environment.
  • Detection of species specific markers for screening of medicinal mushrooms.
  • Biodegradation of contaminants from JK paper Ltd using several microbes and biotechnological process.

2005-06

  • Molecular elucidation of phylogenetic relationship in the genus Cajanus using RAPD & ISSR markers.
  • In-vitro propagation of Jatropha curcas clones through organogenesis and embryogenesis to meet the need of MITS bio-diesel project.
  • Optimization of laboratory scale Ethanol and citric acid production through bioreactor.
  • Genetic fingerprinting of Eucalyptus clones of JK Paper Ltd. using RAPD and ISSR markers.
  • Genetic fingerprinting of Polyanthes tuberosa accession available at Rayagada.
  • In-vitro propagation of Curcuma longa linn.

2006-07

  • Molecular tagging of Flower colour in Asteracantha longifolia using RAPD markers.
  • Molecular tagging of genes controlling white rust in Brassica campestris using BSA.
  • Detection of Sex specific RAPD markers in Momordica dioica.
  • Biomolecular characterization of in-vitro organogenesis in Jatropha curcas using PBPP and isozyme profiling.

2007-08

  • Molecular Characterization of Enteropathogenic isolates of Paediatric patients of MKCG medical college using RAPD, ISSR and PCR-RFLP of 16S-rDNA analysis
  • Micropropagation of Nyctanthes arbortristic and genetic fiedility assessment using RAPD markers.
  • Chromosome number identification and karyotyping of Asteracantha longifolia genome.
  • In-vitro elucidation of antioxidant activities of Annona squamosa leaf extract

Research Publications of the Department:

  • Mishra RR, Behera ML and Panigrahi J (2008) Detection of sex specific RAPD markers in Kakrol. Current Science (In Press)
  • Behera ML, Mishra RR, Bindhani B and Panigrahi J (2008) Chromosomal analysis in Asteracantha longifolia (L.) NEES- a medicinal herb. Cytologia (In Press)
  • Panigrahi J, Behera ML, Maharana SB and Mishra RR (2007) Biomolecular Changes during in vitro organogenesis of Asteracantha longifolia (L.) NEES. Indian J Experimental Biology 45(10): 901-911
  • Panigrahi J, Kumar DR, Mishra M, Mishra RP and Jena P (2007) Genomic relationships in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms. Plant Biotechnology Reports 1 (2): 109-116 ( Springer)
  • Mishra RR, Behera ML, Kumar DR and Panigrahi J (2006) High frequency regeneration of plantlets from seedling explants of Asteracantha longifolia (L.) NEES. J Plant Biotechnology 8 (1): 27-35 ( Springer)
  • Panigrahi J, Mishra RR and Behera ML  (2006) In vitro multiplication of Astercantha longifolia (L.) NEES- a medicinal herb.  Indian J Biotechnology. 5: 562-564 

Workshop and Seminar conducted in the department:

            In order to keep the students acquainted with rapidly changing developments in the field of Biotechnology, the department organizes students and teachers seminar regularly. An oneday workshop-cum seminar is organized on “ Energy plantation & Biodiesel production in Orissa 0n 11th December, 2007.

Quality Improvement Programme for staff:

            Quality improvement activities not only increase the skill and exposure but also update knowledge. Keeping these views in mind, regular training programmes are encouraged for both faculty and students. However, few teachers training programme are enumerated as follows:

  • A 15 days training programme attended by Mr. V.S.S. Rao at JNU, New Delhi on Immunotechniques under the supervision of Prof. Santosh Kar, Co-ordinator Biotechnology.
  • A 10 days training programme om Animal Cell and Tissue culture methods by Mr. V.S.S. Rao at DRDO, New Delhi under the supervision of Dr. Srikanth, Scientist-F.
  • A seven day training programme on Micro array and Plant variety Charaterization by Dr. J. Panigrahi, at NRC on Plant Biotechnology, IARI, New Delhi.
  • A two days workshop on Intellectual property Rights was attended by Dr. J. Panigrahi at NRDC, New Delhi
  • A seven day training programme on “ Engineering aspects of Enzyme and Microbial process” by Mr. R.R. Mishra and Mr. M. L. Behera, at IIT, Guwhati.
  • A two day International seminar-cum-Workshop on “ Lessons from Microbial World” was attended by Mr. G. Sahoo and MR. P. D. Achary at KIIT University, Bhubaneswar.

Study tour Programme for Students:

Students training and projects were conducted either in the department or in the R &D labs of CSIR, ICAR and ICMR. They were also doing industrial projects in various industries including Shanta Biotechnics, Ocimum BIoslutions, Bharath Biotech, Ranbaxy Ltd., Alkem Ltd and Mahyco Seeds Ltd. In addition, following study tour programmes were arranged.

A seven days study tour programme was arranged for  M. Sc. students to Hyderabad and they have visited CCMB, NIN, DRR, NRC for Sorghum, ICRISAT, Bharath Biotech and Shantha Biotechniques.

A seven days study tour programme was arranged for B. Tech students to Bangalore and they have visited Monsanto, Avasthagene, CFTRI, BIOZEEN, MILliPORE, GENEI.

 
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